Job burnout is defined as a syndrome - a complex product of emotional exhaustion, depersonalization and reduced personal achievements that can occur to people who work extensively with many other people under considerable time pressure. Aim: The investigation and measurement of factors contributing in the creation of stress and fatigue of nursing staff so as the satisfaction and self-esteem that nurses live during their professional career. Material and methods: The study population included 167 employees of regular nursing staff working in three public hospitals of the province. Data was collected by completing anonymous structured questionnaire. The program used for entering coded data and statistical processing was SPSS 11.0. Results: Statistical analysis showed that females reported "good" and "very good" emotional status than males with a statistically significant difference, p <0.001. Also, the men surveyed were satisfied with their profession 'often' and 'very often' compared with women with a statistically important difference, p <0.005. Τhe greater the seniority of the respondents the more they say that fatigue at the during the shift. The observed difference is statistically significant at a p level of less than 0.05. Finally there was little statistically significant positive correlation between years of work and fatigue. (p=0,004). Conclusions: Health professionals are more prone to fatigue than other professional workers because they are responsible for human lives and their actions have a significant impact on these people, the workload given to them is very high.