The appropriate compliance of rules regarding hand hygiene, have been and continue to be, one of the basic points of all prevention programs of nosocomial infections. Aim: The aim of the present study was the presentation of guidelines for hand hygiene by nurses. Method and material: Study of international and Greek literature from electronic databases Medline, PubMed and scientific journals, KEELPNO mainly from the last three years. The keywords used were: nosocomial infections, hand hygiene, antiseptics and guidelines. Results: There are numerous studies that demonstrate the central role of hand hygiene of employees in transmission of microorganisms in the hospital environment. Hand hygiene is a process, which should not be omitted or replaced by the use of antiseptics. The professionals position in the hospital, the workplace and staff shortages, in conjunction with the large number of hospitalized patients seem to be the main factors that negatively affect the compliance with hand hygiene. The staff working in the hospital, especially with high-risk patients (ICU, surgery) should not wear artificial nails, nail polish and jewelry - rings and should not have inflammation or infection on their hands. Conclusions: Hand hygiene should be applied before and after contact with each patient. Nurses have to comply with these guidelines, in order for the effective prevention and control of nosocomial infections.