Introduction: The prevention of Central Line-Associated Bloodstream Infections (CLABSIs) can be achieved through systematic surveillance and intervention through the implementation of care bundles. An integral component of the care bundles is the central line insertion and maintenance checklists. Aim: The aim of this literature review was to investigate the compliance rate of the nursing staff with the central line insertion and maintenance care checklists as well as the factors affecting it. Material and Methods: Search was performed on PubMed (April 05, 2021) with the keywords: “nurse*”, “nursing staff”, “compliance”, “central line insertion checklist” and “central line maintenance checklist”, without time limit for the publication of scientific papers. Keywords were searched in the title and abstract of the studies. Studies were excluded if they were not published in the English language, were conducted on animals, and were case studies, editorials, and letters to the editor. Results: Of the scientific papers retrieved, 5 were included in the systematic review. The compliance rate of nursing staff with central line insertion and maintenance checklists ranged from 8.4% to 74.3% and from 11.4% to 77.3%, respectively. Regarding the factors affecting the compliance of nursing staff with both types of checklists, these were the nurse-to-patient ratio and the number of beds of nursing wards. Conclusions: Compliance rates with the central line insertion and maintenance checklists are moderate and low. Interventions are required that will increase compliance rates leading to lower CLABSI rates.